Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
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Invitrogen™ Tris (1 M), pH 7.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
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| Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
|---|---|
| Physical Form | Liquid |
| pH | 7 |
| Grade | Molecular Biology |
| Recommended Storage | Store at room temperature. |
| Concentration | 1 M |
| Product Line | Ambion |
| Color | Undesignated |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-Glycine-SDS |
| Grade | Electrophoresis |
| Identification | Pass Test |
| DNase | DNase free |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 10X Solution |
| Concentration or Composition (by Analyte or Components) | 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS. |
| CAS | 151-21-3 |
| Protease | Protease free |
| Synonym | TG-SDS |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
Hydrochloric acid, 1.0N Standardized Solution
CAS: 7647-01-0 Molecular Formula: ClH Molecular Weight (g/mol): 36.46 MDL Number: MFCD00011324 MFCD00792839 InChI Key: VEXZGXHMUGYJMC-UHFFFAOYSA-N PubChem CID: 313 ChEBI: CHEBI:17883 SMILES: Cl
| PubChem CID | 313 |
|---|---|
| CAS | 7647-01-0 |
| Molecular Weight (g/mol) | 36.46 |
| ChEBI | CHEBI:17883 |
| MDL Number | MFCD00011324 MFCD00792839 |
| SMILES | Cl |
| InChI Key | VEXZGXHMUGYJMC-UHFFFAOYSA-N |
| Molecular Formula | ClH |
Ricca Chemical Company Color Standard, APHA/Hazen (Platinum-Cobalt), Color = 500, Ricca Chemical
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| Name Note | Color= 500 |
|---|---|
| CAS | 7732-18-5 |
| CAS Min % | 0.09 |
| Packaging | Amber Glass Bottle |
| Chemical Name or Material | Color Standard |
| Grade | APHA/Hazen |
| Melting Point | Approximately 0°C |
| CAS Max % | 0.09 |
Fisher Scientific TISAB Concentrate, Fisherbrand
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TE Buffer, Tris-EDTA, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
Tris-EDTA (TE) is routinely used for suspending nucleic acid samples.
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-EDTA |
| Grade | Molecular Biology |
| DNase | DNase free |
| Filtered Through | Filtered through a 5-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 1X Solution, pH 7.4 |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Health Hazard 3 | May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. |
| Health Hazard 2 | CAUTION! |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| Recommended Storage | RT |
Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer (2X)
Ideal for native running conditions with NuPAGE Tris-Acetate gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Hydrochloric acid, 0.1N Standardized Solution
CAS: 7647-01-0 Molecular Formula: ClH Molecular Weight (g/mol): 36.46 MDL Number: MFCD00011324 MFCD00792839 InChI Key: VEXZGXHMUGYJMC-UHFFFAOYSA-N PubChem CID: 313 ChEBI: CHEBI:17883 IUPAC Name: hydrogen chloride SMILES: Cl
| PubChem CID | 313 |
|---|---|
| CAS | 7647-01-0 |
| Molecular Weight (g/mol) | 36.46 |
| ChEBI | CHEBI:17883 |
| MDL Number | MFCD00011324 MFCD00792839 |
| SMILES | Cl |
| IUPAC Name | hydrogen chloride |
| InChI Key | VEXZGXHMUGYJMC-UHFFFAOYSA-N |
| Molecular Formula | ClH |
Ricca Chemical Company Potassium Hydroxide, 0.0100 N (N/100) in Isopropyl Alcohol, Ricca Chemical
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CAS: 1310-58-3 Molecular Formula: HKO Molecular Weight (g/mol): 56.11 MDL Number: MFCD00003553 InChI Key: KWYUFKZDYYNOTN-UHFFFAOYSA-M Synonym: potassium hydroxide,caustic potash,potash lye,potassium hydrate,hydroxyde de potassium,potassa,potasse caustique,potassium hydroxide k oh,potassium hydroxide solution,caswell no. 693 PubChem CID: 14797 ChEBI: CHEBI:32035 IUPAC Name: potassium hydroxide SMILES: [OH-].[K+]
| PubChem CID | 14797 |
|---|---|
| CAS | 1310-58-3 |
| Molecular Weight (g/mol) | 56.11 |
| ChEBI | CHEBI:32035 |
| MDL Number | MFCD00003553 |
| SMILES | [OH-].[K+] |
| Synonym | potassium hydroxide,caustic potash,potash lye,potassium hydrate,hydroxyde de potassium,potassa,potasse caustique,potassium hydroxide k oh,potassium hydroxide solution,caswell no. 693 |
| IUPAC Name | potassium hydroxide |
| InChI Key | KWYUFKZDYYNOTN-UHFFFAOYSA-M |
| Molecular Formula | HKO |
| Linear Formula | KH2PO4 |
|---|---|
| Color | Yellow |
| Traceability to NIST | Traceable to NIST |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution pH 7.0 at 25°C |
| Grade | Certified |
| Identification | Passes Test |
| Name Note | Color Coded Yellow |
| CAS | 1310-73-2 |
| Health Hazard 3 | GHS P Statement If in contact with skin or eyes, rinse thoroughly with water for 15-20 minutes. If swallowed, get medical attention. |
| Decomposition Information | Phosphorus oxides; Sodium oxide |
| Health Hazard 2 | GHS H Statement Solution is not hazardous. |
| Solubility Information | Soluble in water |
| pH | 7.00 ±0.01 |
| Recommended Storage | Room Temperature |
| Formula Weight | 136.09 |
| CAS Max % | 0.08 |
Ricca Chemical Company Zero Oxygen Standard, for Calibration of Dissolved Oxygen Meters, Ricca Chemical
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CAS: 7791-13-1 Molecular Formula: Cl2CoH12O6 Molecular Weight (g/mol): 237.92 MDL Number: MFCD00149652 InChI Key: GFHNAMRJFCEERV-UHFFFAOYSA-L IUPAC Name: λ2-cobalt(2+) hexahydrate dichloride SMILES: O.O.O.O.O.O.[Cl-].[Cl-].[Co++]
| CAS | 7791-13-1 |
|---|---|
| Molecular Weight (g/mol) | 237.92 |
| MDL Number | MFCD00149652 |
| SMILES | O.O.O.O.O.O.[Cl-].[Cl-].[Co++] |
| IUPAC Name | λ2-cobalt(2+) hexahydrate dichloride |
| InChI Key | GFHNAMRJFCEERV-UHFFFAOYSA-L |
| Molecular Formula | Cl2CoH12O6 |
Tetrahydrofuran Anhydrous (stabilized with BHT) 99.5+%, TCI America™
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CAS: 109-99-9 Molecular Formula: C4H8O Molecular Weight (g/mol): 72.11 MDL Number: MFCD00005356 InChI Key: WYURNTSHIVDZCO-UHFFFAOYSA-N Synonym: tetrahydrofuran,furan, tetrahydro,butylene oxide,furanidine,hydrofuran,tetramethylene oxide,oxacyclopentane,1,4-epoxybutane,tetrahydrofuranne,tetraidrofurano PubChem CID: 8028 ChEBI: CHEBI:26911 IUPAC Name: oxolane SMILES: C1CCOC1
| PubChem CID | 8028 |
|---|---|
| CAS | 109-99-9 |
| Molecular Weight (g/mol) | 72.11 |
| ChEBI | CHEBI:26911 |
| MDL Number | MFCD00005356 |
| SMILES | C1CCOC1 |
| Synonym | tetrahydrofuran,furan, tetrahydro,butylene oxide,furanidine,hydrofuran,tetramethylene oxide,oxacyclopentane,1,4-epoxybutane,tetrahydrofuranne,tetraidrofurano |
| IUPAC Name | oxolane |
| InChI Key | WYURNTSHIVDZCO-UHFFFAOYSA-N |
| Molecular Formula | C4H8O |
Ricca Chemical Company Sodium Thiosulfate, 0.100 N (N/10), Ricca Chemical
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CAS: 10102-17-7 Molecular Formula: H10Na2O8S2 InChI Key: PODWXQQNRWNDGD-UHFFFAOYSA-L Synonym: sodium thiosulfate pentahydrate,ametox,sodium thiosulfate, pentahydrate,antichlor,tinver,disodium thiosulfate pentahydrate,unii-hx1032v43m,ccris 3952,thiosulfuric acid, disodium salt, pentahydrate,sodium hyposulfite pentahydrate PubChem CID: 61475 ChEBI: CHEBI:32150
| PubChem CID | 61475 |
|---|---|
| CAS | 10102-17-7 |
| ChEBI | CHEBI:32150 |
| Synonym | sodium thiosulfate pentahydrate,ametox,sodium thiosulfate, pentahydrate,antichlor,tinver,disodium thiosulfate pentahydrate,unii-hx1032v43m,ccris 3952,thiosulfuric acid, disodium salt, pentahydrate,sodium hyposulfite pentahydrate |
| InChI Key | PODWXQQNRWNDGD-UHFFFAOYSA-L |
| Molecular Formula | H10Na2O8S2 |
Ricca Chemical Company Buffer, Reference Standard, pH 11.00 ± 0.01 at 25°C, Ricca Chemical
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| For Use With (Equipment) | pH Meter and Electrode |
|---|---|
| Physical Form | Liquid |
| pH | 11 |
| CAS Min % | 0.11 |
| Concentration | 1X |
| For Use With (Application) | Calibration |
| Solution Type | pH Buffer |
| CAS Max % | 0.11 |
Ricca Chemical Company Buffer, Reference Standard, pH 12.00 ± 0.01 at 25°C, Ricca Chemical
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| Boiling Point | 100°C |
|---|---|
| Color | Colorless |
| Physical Form | Liquid |
| CAS Min % | 0.08 |
| Chemical Name or Material | Buffer, Reference Standard |
| Grade | Reference Standard |
| Concentration | 1X |
| For Use With (Application) | Calibration |
| Sterility | Non-sterile |
| Name Note | pH 12.00± 0.01 at 25°C |
| CAS | 7732-18-5 |
| For Use With (Equipment) | pH Meter and Electrode |
| Solubility Information | Miscible |
| pH | 12 |
| DOT Information | Not Regulated by DOT |
| Shelf Life | 18 months |
| Solution Type | pH Buffer |
| CAS Max % | 0.08 |
| Melting Point | 0°C |